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eBook/Digital Version available from:
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Score: 89 |
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| Animal Coronaviruses |
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ISBN: 978-1-4939-3412-6,
218 pages,
Hard Cover
ISBN-10: 1-4939-3412-0 |
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| Copyright: |
2016 |
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| Edition: |
1st |
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| Editor: |
Wang, Leyi |
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Specialties:
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Veterinary Medicine
, Virology |
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| Publisher: |
Springer |
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| Imprint: |
Humana Press |
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| Series Title: |
Springer Protocols |
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| List Price: |
$94.99 |
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| Google: |
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At A Glance
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This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.
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Reviewer:
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Dongwan Yoo,
DVM, PhD
(University of Illinois College of Veterinary Medicine)
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Range
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Question
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Score
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| 1-10 |
Are the author's objectives met? |
10 |
| 1-10 |
Rate the worthiness of those objectives. |
8 |
| 1-5 |
Is this written at an appropriate level? |
5 |
| 1-5 |
Is there significant duplication? (1=significant, 5=insignificant) |
5 |
| 1-5 |
Are there significant omissions? (1=significant, 5=insignificant) |
3 |
| 1-5 |
Rate the authority of the authors. |
5 |
| 1-5 |
Are there sufficient illustrations? |
5 |
| 1-5 |
Rate the pedagogic value of the illustrations. |
4 |
| 1-5 |
Rate the print quality of the illustrations. |
4 |
| 1-5 |
Are there sufficient references? |
5 |
| 1-5 |
Rate the currency of the references. |
5 |
| 1-5 |
Rate the pertinence of the references. |
4 |
| 1-5 |
Rate the helpfulness of the index. |
3 |
| 1-5 |
If important in this specialty, rate the physical appearance of the book |
N/A |
| 1-10 |
Is this a worthwhile contribution to the field? |
10 |
| 1-10 |
If this is a 2nd or later edition, is this new edition needed? |
N/A |
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Reviewer:
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Dongwan Yoo,
DVM, PhD
(University of Illinois College of Veterinary Medicine)
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Description
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| Coronaviruses infect a wide range of animal species, including birds and humans, and are important veterinary pathogens causing significant economic losses worldwide. Novel coronaviruses have been identified in the past several decades, and they have drawn significant attention as emerging viruses. This book is the first to illustrate experimental and diagnostic protocols for various animal coronaviruses. Both a hard copy and an online version are available. |
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Purpose
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| This book is unique in presenting current technologies available for detection of animal coronaviruses and research especially for diagnostic methodologies. The intention is to provide specific protocols and methods for individual coronaviruses, both conventional approaches and state-of-the art technologies. It presents a total of 19 protocols, and many of the contributors are diagnosticians who handle clinical specimens on a daily basis in diagnostic laboratories. |
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Audience
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| The primary audience is diagnosticians, interns, and residents who work in the laboratory, but graduate students and researchers will benefit as well. |
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Features
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| The book covers both standard virological techniques and molecular techniques for animal coronaviruses. Notable additions are the protocols for detection of equine coronavirus and ferret coronavirus, both recently identified. Emerging viruses in pigs, porcine epidemic diarrhea virus and porcine deltacoronavirus are also included. The differential diagnostic test for feline coronavirus and infectious peritonitis virus can be a problematic, but the protocol in this book will be a useful guide for clinicians and diagnosticians. The chapters for reverse genetics approaches to avian infectious coronavirus and feline coronavirus will also be useful for researchers in the field. The application of next generation sequencing techniques to whole genome sequencing of porcine coronaviruses in the fecal samples are particularly interesting and will be useful for beginners to adapt such an approach to research and diagnostic developments. |
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Assessment
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| Typically, books on coronavirus are collections of conference presentations, reviews, and/or molecular biology, but this book presents specific laboratory protocols for detection of individual viruses and applications to coronavirus research. Various recently identified animal coronaviruses are included; human coronaviruses are excluded. This book is essential for researchers and diagnosticians in veterinary virology. |
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